IL-4R alpha/CD124 蛋白, Cynomolgus (HEK293, His)

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation^[1]. IL-4R alpha/CD124, Cynomolgus consists of 825 amino acids (M1-S825). IL-4R alpha/CD124 Protein, Cynomolgus (M26-R232) is produced in HEK293 cells, including a Fibronectin typr III domain (125-224 a.a) and a C-Terminal His-tag.

白介素 4R α (IL-4Rα) 也称为 CD124 和 B 细胞刺激因子 (BSF) 受体，是一种抗炎细胞因子，在活化的 T 细胞中高表达^[1]。
IL-4R α 参与不同细胞类型中两种白细胞介素受体的形成。对于I型受体，依赖于IL-4Rα结合IL-4来招募免疫细胞中的IL-2Rγ链。IL-2R γ 是多种白细胞介素受体的常见亚基，参与 IL-15 刺激中性粒细胞的吞噬作用。对于II型受体，依赖IL-4Rα结合IL-4来募集IL-13Rα1链。IL-13R α 1 是非免疫细胞中常见细胞因子受体 γ 链的替代辅助蛋白^[2][3]。
IL-4R α蛋白中的氨基酸在不同动物之间有很大差异。
IL-4 R α通过交替剪接或蛋白水解产生可溶形式，保持配体结合特性并抑制IL-4生物活性。IL-4 R α 可溶性亚型 1 可以通过金属蛋白酶在细胞表面进行蛋白水解裂解（脱落）来产生^[4]。
IL-4 R α 在 Th2 中发挥重要作用- 免疫反应偏向、巨噬细胞活化、粘膜免疫、过敏性炎症、肿瘤进展和动脉粥样硬化^[5]。

IL-4Rα is regulated by variant IL-4 (VIL-4), produced by Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques, and induces expansion of Vgamma2Vdelta2 T cells^[1]

IL-4Rα is regulated by variant IL-4 (VIL-4), produced by Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques, and induces expansion of Vgamma2Vdelta2 T cells^[6]

Cynomolgus

HEK293

C-6*His

G7Q0S7 (M26-R232)

/

MKVLQEPACVSDYMSISTCEWKMGGPTNCSAELRLLYQLVFQSSETHTCVPENNGGVGCVCHLLMDDVVSMDNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTVLLTWSNPYPPDNYLYNDLTYAVNIWSENDPAYSRIHNVTYLKPTLRIPASTLKSGISYRARVRAWAQHYNTTWSEWSPSTKWYNSYREPFEQR

38-58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Keegan AD,et al. An IL-4 receptor region containing an insulin receptor motif is important for IL-4-mediated IRS-1 phosphorylation and cell growth. Cell. 1994 Mar 11;76(5):811-20.  [Content Brief]

  [2]. Zurawski SM, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995 Jun 9;270(23):13869-78.  [Content Brief]

  [3]. Rolling C, et al. IL4 and IL13 receptors share the gamma c chain and activate STAT6, STAT3 and STAT5 proteins in normal human B cells. FEBS Lett. 1996 Sep 9;393(1):53-6.  [Content Brief]

  [4]. Jung T, et al. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999 May;119(1):23-30.  [Content Brief]

  [5]. HageT,etal.Crystalstructureoftheinterleukin-4/receptoralphachaincomplexrevealsamosaicbindinginterface.Cell.1999Apr16;97(2):271-81.  [Content Brief]

  [6]. Yuan Z, et al. Tuberculosis-induced variant IL-4 mRNA encodes a cytokine functioning as growth factor for (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-specific Vgamma2Vdelta2 T cells. J Immunol. 2009 Jan 15;182(2):811-9.  [Content Brief]