IL-5 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

IL-5 protein is expressed by T lymphocytes and NK cells and regulates eosinophils, affecting their survival, differentiation and chemotaxis.In addition, IL-5 stimulates immunoglobulin production, growth, and differentiation of activated and resting B cells.IL-5 Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived IL-5 protein, expressed by HEK293 , with N-His, N-Avi labeled tag.

IL-5 蛋白是一种同型二聚体细胞因子，主要由 T 淋巴细胞和 NK 细胞表达，通过影响嗜酸性粒细胞的存活、分化和趋化性，在调节嗜酸性粒细胞中发挥关键作用。此外，IL-5 作用于激活的和静止的 B 细胞，刺激免疫球蛋白的产生、生长和分化。从机制上讲，IL-5的生物学效应是通过由IL5RA亚基和细胞因子受体共同亚基β/CSF2RB组成的受体复合物介导的。与受体结合后，IL-5 会触发各种激酶的激活，包括 LYN、SYK 和 JAK2，从而通过 RAS-MAPK 和 JAK-STAT5 途径传播信号（通过相似性）。在结构上，IL-5 形成二硫键连接的同二聚体，并与其受体成分 IL5RA 和 CSF2RB 相互作用，突出了其分子相互作用在协调免疫反应中的特异性和复杂性。

Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His Avi Tag with an affinity constant of 0.511 μM as determined in SPR assay (Biacore T200).

Mouse

HEK293

N-His;N-Avi

P04401 (M21-G133)

16191  [NCBI]

16 kDa&20-35 kDa

Greater than 95% as determined by Tris-Bis PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.