2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Peptide Hormone & Neuropeptides
  5. Insulin
  6. Insulin-1/Ins1 蛋白, Mouse (P.pastoris, Myc, His, solution)

Insulin-1/Ins1 蛋白, Mouse (P.pastoris, Myc, His, solution)

目录号: HY-P71805Y
COA 产品使用指南

Insulin-1 是葡萄糖的重要调节剂，可以通过增加细胞对单糖、氨基酸和脂肪酸的通透性来有效降低血糖。其作用包括加速糖酵解、促进磷酸戊糖循环、促进肝糖原合成。Insulin-1/Ins1 蛋白, Mouse (P.pastoris, Myc, His, solution) 是重组的 胰岛素 Ins1 蛋白，由 P. pastoris 表达，带有 N-His, C-Myc 标签。

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μg ￥5650 In-stock
50 μg   询价  
100 μg   询价  

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描述

Insulin-1 is an important regulator of glucose and can effectively lower blood sugar by increasing cellular permeability to simple sugars, amino acids, and fatty acids.Its effects include accelerating glycolysis, promoting pentose phosphate cycle, and promoting hepatic glycogen synthesis.Insulin-1/Ins1 Protein, Mouse (P.pastoris, Myc, His, solution) is the recombinant mouse-derived Insulin-1/Ins1 protein, expressed by P.pastoris , with N-His, C-Myc labeled tag.
研究背景

胰岛素-1 是血糖调节的关键角色，通过增强细胞对单糖、氨基酸和脂肪酸的通透性，有效降低血糖水平。其多方面作用包括加速糖酵解、促进磷酸戊糖循环以及促进肝脏中糖原的合成。在结构上，Insulin-1 以异二聚体形式存在，包含 B 链和 A 链，通过两个二硫键错综复杂地连接。这些结构特征强调了其在协调维持葡萄糖稳态所必需的代谢过程中的关键作用。
种属

Mouse
表达系统

P. pastoris
标签

N-His;C-Myc
蛋白编号

P01325 (F25-N108)
基因 ID

16333  [NCBI]
蛋白结构
N-term
Ins1 (F25-N18)
Accession # P1325
Myc
C-term
同用名
Ins1; Ins-1; Insulin-1
氨基酸序列

FVKQHLCGPHLVEALYLVCGERGFFYTPKSRREVEDPQVEQLELGGSPGDLQTLALEVARQKRGIVDQCCTSICSLYQLENYCN

分子量

Approximately 25 kDa
纯度

Greater than 90 % as determined by SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, 20% Glycerol.
内毒素含量

<1 EU/ug, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (208 KB)

产品使用指南 (1213 KB)

Insulin-1/Ins1 Protein, Mouse (P.pastoris, Myc, His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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