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  3. KBTBD2 蛋白, Human (Sf9)

KBTBD2 蛋白, Human (Sf9)

目录号: HY-P701575
产品使用指南

BCR E3 泛素连接酶复合物中的 KBTBD2 是胰岛素信号通路的关键调节因子,影响脂肪细胞的胰岛素敏感性。其作用通过协调 PIK3R1 降解的泛素化过程来调节 PIK3R1/p85alpha 丰度来体现。KBTBD2 蛋白, Human (Sf9) 是重组的 KBTBD2 蛋白,由 Sf9 insect cells 表达,不带标签。KBTBD2 蛋白, Human (Sf9) 全长 622 个氨基酸。

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描述

KBTBD2 in the BCR E3 ubiquitin ligase complex is a key regulator in the insulin signaling pathway and affects the insulin sensitivity of adipocytes. Its effects are manifested by regulating PIK3R1/p85alpha abundance by coordinating the ubiquitination process for PIK3R1 degradation. KBTBD2 Protein, Human (Sf9) is the recombinant human-derived KBTBD2 protein, expressed by Sf9 insect cells , with tag free. The total length of KBTBD2 Protein, Human (Sf9) is 622 a.a., .

研究背景

KBTBD2 是 BCR (BTB-CUL3-RBX1) E3 泛素连接酶复合物内的底物特异性接头,是胰岛素信号通路中的关键调节因子,对脂肪细胞的胰岛素敏感性具有显着影响。这种调节作用是通过调节 PIK3R1/p85alpha 丰度(磷脂酰肌醇 3-激酶 (PI3K) 的调节亚基)来执行的。值得注意的是,KBTBD2 协调针对 PIK3R1 的泛素化过程,以实现“Lys-48”连接的多泛素化,从而导致其随后的蛋白酶体介导的降解。KBTBD2 精心策划的复杂蛋白质修饰机制,特别是其在蛋白质泛素化中的作用,强调了其在微调胰岛素信号级联以及脂肪细胞内胰岛素敏感性方面的关键功能。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q8IY47 (S2-V623)

基因 ID

25948

蛋白结构
N-term
KBTBD2 (S2-V623)
Accession # Q8IY47
C-term
同用名
KBTBD2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

KBTBD2 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
KBTBD2 Protein, Human (Sf9)
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HY-P701575
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