KRAS 蛋白, Human (G13C, His)

KRAS protein is a key Ras family member that binds GDP/GTP and has intrinsic GTPase activity. Its critical role in regulating cell proliferation emphasizes its importance in cellular processes. KRAS Protein, Human (G13C, His) is the recombinant human-derived KRAS protein, expressed by E. coli , with N-6*His labeled tag and G13C, , , , mutation. The total length of KRAS Protein, Human (G13C, His) is 184 a.a., with molecular weight of ~25 kDa.

KRAS 是 Ras 蛋白家族的关键成员，具有结合 GDP/GTP 的能力，并具有内在的 GTP 酶活性。它对细胞增殖调节的重要参与强调了它在细胞过程中的重要性。值得注意的是，KRAS 在促进致癌事件中发挥着重要作用，特别是在结直肠癌 (CRC) 中，它以 ZNF304 依赖性方式诱导肿瘤抑制基因 (TSG) 的转录沉默。这种多方面的功能凸显了 KRAS 作为细胞动力学的关键参与者，并强调了它在正常和病理细胞过程中的相关性。

Measured by its ability to catalyze the substrate GTP. The specific activity is 2.08 nmol/min/mg, as measured under the described conditions.

Human

E. coli

N-6*His

AAH13572.1 (T2-C185, G13C)

3845  [NCBI]

TEYKLVVVGAGCVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC

Approximately 25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.