LILRB2/CD85d/ILT-4 蛋白, Human (HEK293, His)

The LILRB2/CD85d/ILT-4 protein is a receptor for class I MHC antigens and can recognize multiple HLA alleles. It crucially downregulates the immune response and builds tolerance. LILRB2/CD85d/ILT-4 Protein, Human (HEK293, His) is the recombinant human-derived LILRB2/CD85d/ILT-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LILRB2/CD85d/ILT-4 Protein, Human (HEK293, His) is 437 a.a., with molecular weight of 58-75 kDa.

LILRB2/CD85d/ILT-4 蛋白作为 I 类 MHC 抗原的受体，可广泛识别 HLA-A、HLA-B、HLA-C、HLA-G 和 HLA-F 等位基因。它在免疫反应下调和耐受性建立中起着至关重要的作用。具体来说，它识别与 B2M/β-2 微球蛋白和九聚体自肽复合的 HLA-G，导致 1 型调节性 T 细胞和骨髓源性抑制细胞的分化，这对于维持母胎耐受性至关重要。LILRB2 与 CD8A 竞争与 I 类 MHC 抗原的结合，并抑制 FCGR1A 介导的细胞反应，包括蛋白质磷酸化和细胞内钙离子的动员。此外，它在磷酸化时与 PTPN6 相互作用并与 FCGR1A 结合。与肽结合的 HLA-G-B2M 和 HLA-F-B2M 的直接相互作用进一步凸显了其参与免疫调节。

Human

HEK293

C-6*His

AAH36827.1 (Q22-H458)

10288  [NCBI]

QTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAYNLSSEWSAPSDPLDILITGQIHGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRH

58-75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.