MCAD 蛋白, Human (His)

MCAD proteins play a key role in mitochondrial fatty acid beta-oxidation, initiating the breakdown of fatty acids into acetyl-CoA for energy production. Specifically, MCAD catalyzes the removal of hydrogen from C-2 and C-3 of linear fatty acyl-CoA thioesters to form trans-2-enoyl-CoA. MCAD Protein, Human (His) is the recombinant human-derived MCAD protein, expressed by E. coli , with N-His labeled tag. The total length of MCAD Protein, Human (His) is 396 a.a., with molecular weight of ~42.0 kDa.

MCAD 蛋白作为参与线粒体脂肪酸 β 氧化初始阶段的酰基辅酶 A 脱氢酶之一发挥着关键作用，线粒体脂肪酸 β 氧化是一个重要的有氧过程，可将脂肪酸分解为乙酰辅酶A，从而能够从脂肪中产生能量。在此过程中，MCAD 催化直链脂肪酰基辅酶 A 硫酯的 C-2 和 C-3 上的氢脱除，从而形成反式 2-烯酰辅酶A。随后，电子受体电子转移黄素蛋白 (ETF) 促进电子向主要线粒体呼吸链的转移，而 ETF 又将电子传递给 ETF-泛醌氧化还原酶（ETF 脱氢酶）。值得注意的是，MCAD 对饱和主链长度为 6 至 12 个碳的酰基辅酶 A 表现出特异性。这些分子事件的复杂编排强调了 MCAD 在代谢途径中不可或缺的作用，有助于有效利用脂肪酸作为线粒体的能量来源。

Human

E. coli

N-His

P11310 (K26-N421)

34  [NCBI]

HHHHHHKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Approximately 42.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris, 100 mM NaCl, 20% Glycerol, pH 8.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. K Tanaka,et al. Mutations in the Medium Chain acyl-CoA Dehydrogenase (MCAD) Gene. Hum Mutat. 1992;1(4):271-9.  [Content Brief]