MCP-1/CCL2 蛋白, Rat (HEK293, C-His)

MCP-1/CCL2 Protein, Rat (HEK293, His) is a small cytokine that belongs to the CC chemokine family.

CCL2 由多种细胞类型产生，可以是组成型的，也可以是在氧化应激、细胞因子或生长因子诱导后产生的。CCL2 由许多细胞类型产生，包括内皮细胞、成纤维细胞、上皮细胞、平滑肌细胞、系膜细胞、星形细胞、单核细胞和小胶质细胞。CCL2 调节单核细胞、记忆 T 淋巴细胞和自然杀伤 (NK) 细胞的迁移和浸润^[1]。趋化因子 CCL2 及其主要趋化因子受体 CCR2 与多种不同疾病过程的发病机制有关，包括转移过程中的血管通透性和免疫细胞的吸引力、多种不同的神经系统疾病、自身免疫性疾病、肥胖和动脉粥样硬化^[2]。

CCL2 (s.c., 4 or 8 μg/kg, daily, 5 days) has no effect on food intake, water intake, weight, or spontaneous abortion while increases CCL2/CCR2 and melanin-concentrating hormone (MCH) expression and cell density in progeny lateral hypothalamus in pregnant rats. Also, CCL2 increases ethanol intake and anxiety-like behavior and decreases motor activity in female and male adolescent offspring, and this effect is significantly stronger in females^[4].

The ED₅₀ is <0.3 μg/mL as measured by CHO-K1/Gα15/rCCR2/THP-1 cells (human Gα15 and rat CCR2 stably expressed in CHO-K1 cells).

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED₅₀for this effect is 5.648 ng/mL, corresponding to a specific activity is 1.771×10⁵ U/mg.

Rat

HEK293

C-6*His

P14844 (Q24-N148)

24770  [NCBI]

QPDAVNAPLTCCYSFTGKMIPMSRLENYKRITSSRCPKEAVVFVTKLKREICADPNKEWVQKYIRKLDQNQVRSETTVFYKIASTLRTSAPLNVNLTHKSEANASTLFSTTTSSTSVEVTSMTEN

Approximately 27-43 kDa under reducing SDS-PAGE due to glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Qiongyu Hao, et al.  CCL2/CCR2 signaling in cancer pathogenesis. Cell Commun Signal. 2020 May 29;18(1):82.  [Content Brief]

  [2]. Deshmane SL, et al. Monocyte chemoattractant protein-1 (MCP-1): an overview. J Interferon Cytokine Res. 2009 Jun;29(6):313-26.  [Content Brief]

  [3]. Svetlana M Stamatovic, et al. Monocyte chemoattractant protein-1 regulation of blood-brain barrier permeability. J Cereb Blood Flow Metab. 2005 May;25(5):593-606.  [Content Brief]

  [4]. O'Connor T, et al. CCL2-CCR2 Signaling in Disease Pathogenesis. Endocr Metab Immune Disord Drug Targets. 2015;15(2):105-18.  [Content Brief]

  [5]. Rachel N Gomes, et al. Bacterial clearance in septic mice is modulated by MCP-1/CCL2 and nitric oxide. Shock. 2013 Jan;39(1):63-9.  [Content Brief]

  [6]. Guo-Qing Chang, et al. Hypothalamic CCL2/CCR2 Chemokine System: Role in Sexually Dimorphic Effects of Maternal Ethanol Exposure on Melanin-Concentrating Hormone and Behavior in Adolescent Offspring. J Neurosci. 2018 Oct 17;38(42):9072-9090.  [Content Brief]