MDM4/MDMX 蛋白, Human (His)

MDM4/MDMX proteins cooperate with MDM2 to regulate TP53 (p53) and inhibit the transcriptional activation domains of p53 and p73. This hinders their ability to induce cell cycle arrest and apoptosis. MDM4/MDMX Protein, Human (His) is the recombinant human-derived MDM4/MDMX protein, expressed by E. coli , with N-His labeled tag.

MDM4/MDMX 蛋白与 MDM2 协同调节 TP53 (p53)。它通过抑制 p53 和 p73 的转录激活结构域发挥作用，从而阻碍它们诱导细胞周期停滞和凋亡的能力。此外，MDM4 阻碍 MDM2 的降解，并可以逆转 MDM2 介导的 TP53 降解，同时抑制 TP53 反式激活和凋亡功能。该蛋白与 MDM2 和 USP2 形成三聚体复合物，并与 TP53、TP73 和 USP2 相互作用。值得注意的是，当磷酸化时，MDM4 与 YWHAG 相互作用，对其对 TP53 的活性产生负调节作用。这些相互作用凸显了 MDM4 在调节 TP53 通路关键成分中的复杂作用，有助于精细调节细胞对压力和 DNA 损伤的反应。

Measured by its ability to promote proliferation of A549 human non small cell lung cancer cell. The ED50 for this effect is 33.7 ng/mL, corresponding to a specific activity is 2.967×10⁴ units/mg.

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  Measured by its ability to promote proliferation of A549 human non small cell lung cancer cell. The ED₅₀ for this effect is 33.7 ng/mL , corresponding to a specific activity is 2.967×10⁴ units/mg.

Human

E. coli

N-His

O15151-1/NP_002384.2 (M1-D134)

4194  [NCBI]

MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQD

Approximately 19.4 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.