METTL11A 蛋白, Human (His)

The METTL11A protein methylates the N-terminus of target proteins, specifically catalyzing monomethylation, dimethylation, or trimethylation of exposed α-amino groups in the [Ala/Gly/Ser]-Pro-Lys motif.METTL11A Protein, Human (His) is the recombinant human-derived METTL11A protein, expressed by E.coli , with N-6*His labeled tag.

METTL11A 蛋白作为分布式 α-N-甲基转移酶，具有在起始子裂解后甲基化含有特定 N 端基序 [Ala/Gly/Pro/Ser]-Pro-Lys 的目标蛋白 N 端的能力蛋氨酸。这种酶活性导致[Ala/Gly/Ser]-Pro-Lys基序中暴露的Ala、Gly或Ser残基的α-氨基的单甲基化、二甲基化或三甲基化，以及单甲基化。或 Pro-Pro-Lys 基序中 Pro 的二甲基化。一些底物可能会通过 NTMT2 介导的单甲基化而引发。METTL11A 负责去除 Met-1 后 CENPA 中 N 端甘氨酸的三甲基化，并在多种蛋白质的 N 端甲基化中发挥至关重要的作用，包括 KLHL31、MYL2、MYL3、RB1、RCC1、RPL23A 和放。在有丝分裂过程中，METTL11A 是正常双极纺锤体形成和染色体分离所必需的，通过作用于 RCC1 发挥其作用。这些不同的甲基化活性凸显了 METTL11A 在调节关键细胞过程和蛋白质功能中的作用。

Measured in a cell proliferation assay using SiHa cells. The ED₅₀ for this effect is 12.43 ng/mL, corresponding to a specific activity is 8.05×10⁴ units/mg.

Human

E. coli

N-6*His

Q9BV86-1 (T2-R223)

28989

TSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 150 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.