NCL 蛋白, Human (P.pastoris, His)

Nucleolar protein (NCL) is the major nucleolar protein in actively growing eukaryotic cells and is associated with intranucleolar chromatin and preribosomal granules. It induces chromatin decondensation by binding to histone H1 and plays a role in pre-rRNA transcription, ribosome assembly, and potential transcription elongation. NCL Protein, Human (P.pastoris, His) is the recombinant human-derived NCL protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of NCL Protein, Human (P.pastoris, His) is 481 a.a., with molecular weight (post-translational modification form) of ~72 KDa.

核仁蛋白 (NCL) 是活跃生长的真核细胞中的主要核仁蛋白，与核仁内染色质和前核糖体颗粒相关。通过与组蛋白 H1 结合，促进其参与诱导染色质解缩。核仁素参与前 rRNA 转录、核糖体组装，并可能在转录延伸中发挥作用。与具有 5'-TTAGGG-3' 重复的端粒单链 DNA 相比，它对包含 5'-UUAGGG-3' 重复的 RNA 寡核苷酸表现出更高的亲和力。此外，NCL 在 IGF2BP1 依赖性 mRNP 颗粒复合物中被鉴定。它是 SWAP 复合体的一部分，也是一个涉及 HTATSF1、CDK9、CCNT1、RNA 聚合酶 II、SUPT5H 和 Nucleolin 的更大复合体的一部分。NCL 与 AICDA、APTX、C1QBP、ERBB4、FMR1、GZF1、NSUN2、NVL、SETX、TERT、WDR46、ZFP36、LRRC34、RRP1B、HNRNPU、RIOK1、ZBTB7B、MDK 和 HDGF 等蛋白质进行多种相互作用，突出了其在各种细胞过程中发挥多方面的作用。

Human

P. pastoris

N-6*His

P19338 (V2-S482)

4691  [NCBI]

VKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWS

Approximately 72 kDa. The reducing (R) protein migrat es as 72 kDa in SDS-PAGE may be due to post-translational modification.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 or 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.