Neprilysin/CD10 蛋白, Rhesus Macaque (HEK293, His)

MME is a cell-surface peptidase member of the M13 family of zinc peptidases, which also includes endothelin converting enzymes (ECE-1 and ECE-2), KELL and PEX. MME cleaves peptide bonds on the amino side of hydrophobic amino acids and is the key enzyme in processing of a variety of physiologically active peptides, such as GRP, neurotensin (NT), and vasoactive intestinal peptide (VIP). MME is downregulated in nearly 50% of primary and metastatic prostate cancers. Neprilysin/CD10 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Neprilysin/CD10 protein, expressed by HEK293 , with N-His labeled tag. The total length of Neprilysin/CD10 Protein, Rhesus Macaque (HEK293, His) is 699 a.a., with molecular weight of ~82.1 kDa.

MME 是锌肽酶 M13 家族中的一种细胞表面肽酶，它还包括内皮素转换酶 (ECE-1和ECE-2)、KELL和PEX。MME 可裂解疏水氨基酸的氨基端肽键，是加工多种生理活性肽如 GRP、神经降压素 (neurotensin, NT) 和血管活性肠肽 (vasoactive intestinal peptide, VIP) 的关键酶。在近 50% 的原发性和转移性前列腺癌中，MME 表达下调，可独立预测较差的预后。除了雄激素撤退下调 MME 的表达外，甲基化也下调 MME 的表达，提示其具有肿瘤抑制作用。事实上，在细胞培养中，MME 的表达降低了前列腺癌细胞的生长、运动和生存。与这些观察结果一致，在异种移植实验中，替代 MME 抑制前列腺癌细胞的致瘤性^[1]。

Measured by its ability to cleave a peptide substrate, Mca-(ala7,lys(dnp)9)-bradykinin. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 4008.09 pmoL/min/µg, as measured under the described conditions.

Rhesus Macaque

HEK293

N-His

F7H3Y6 (Y52-W750)

707667  [NCBI]

YDDGICKSSDCIKSAARLIQNMDATAEPCADFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQLQSNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Approximately 95 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.