Neuritin 蛋白, Human (N-His)

Neuritin, a pivotal factor in neural development, significantly enhances neurite outgrowth and branching in hippocampal and cortical cells. Within the AMPA receptor (AMPAR) complex, Neuritin contributes to the outer core, modulating the function and properties of these receptors. The outer core, involving specific interactions with Neuritin, PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L, and NRN1, plays a crucial role in fine-tuning the gating, pharmacology, biogenesis, and protein processing of AMPARs. Neuritin's regulatory role in this protein-protein network underscores its significance in shaping AMPAR functional properties and neuronal connectivity. Neuritin Protein, Human (N-His) is the recombinant human-derived Neuritin protein, expressed by E. coli, with N-6*His labeled tag. The total length of Neuritin Protein, Human (N-His) is 88 a.a., with molecular weight of ~11 kDa.

神经突蛋白是神经发育的关键参与者，可显着增强原代海马和皮质细胞的神经突生长和分支。在 AMPA 受体 (AMPAR) 复合物中，神经蛋白有助于形成外核，而外核是调节这些受体功能和特性的复杂结构的一部分。AMPAR 复合体由具有成孔 GluA/GRIA 蛋白和主要辅助亚基的内核组成，涉及与 Neuritin 以及外核中的其他成分（如 PRRT1、PRRT2、CKAMP44/SHISA9、FRRS1L 和 NRN1）的特异性相互作用。这个外核对于微调 AMPAR 的门控、药理学、生物发生和蛋白质加工至关重要。Neuritin 参与蛋白质-蛋白质相互作用的复杂网络，凸显了其作为调节元件在塑造 AMPAR 功能特性中的作用，并强调了其在神经元连接中的重要性。

Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ this effect is 17.37 ng/ml, corresponding to a specific activity is 5.76×10^4 units/mg.

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  Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ this effect is 17.37 ng/mL, corresponding to a specific activity is 5.76×10⁴ units/mg.

Human

E. coli

N-6*His

Q9NPD7 (A28-N115)

51299  [NCBI]

AGKCDAVFKGFSDCLLKLGDSMANYPQGLDDKTNIKTVCTYWEDFHSCTVTALTDCQEGAKDMWDKLRKESKNLNIQGSLFELCGSGN

Approximately 11 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.