PPIE 蛋白, Human (His)

The PPIE protein is a key spliceosome component that complexly regulates pre-mRNA splicing through RNA binding and PPIase activity. It prefers single-stranded RNA with polyA and polyU stretches, indicating an affinity for the poly(A) region of the 3'-UTR. PPIE Protein, Human (His) is the recombinant human-derived PPIE protein, expressed by E. coli , with N-6*His labeled tag. The total length of PPIE Protein, Human (His) is 301 a.a., with molecular weight of ~34.0 kDa.

PPIE 蛋白作为剪接体的组成部分，在前 mRNA 剪接中发挥着至关重要的作用。这种多功能蛋白结合了 RNA 结合和肽基脯氨酰顺反异构酶 (PPIase) 活性，有助于复杂的剪接调节过程。PPIE 表现出对具有 Poly-A 和 Poly-U 延伸的单链 RNA 分子的偏好，表明其对 mRNA 分子 3'-UTR 中的 Poly(A) 区域的亲和力。除了在 RNA 结合中的作用外，PPIE 还催化蛋白质中脯氨酸酰亚胺肽键的顺反异构化。值得注意的是，它作为 KMT2A 活性的抑制剂发挥作用，并且这种调节作用取决于其脯氨酸异构酶活性。PPIE 的多功能功能强调了其在协调细胞过程各个方面（包括 RNA 剪接和蛋白质构象变化）方面的重要性。

Measured by its ability to convert the 0.3mM substrate of Suc-AAPF-pNA, from Cis to Transformation. The specific activity is 4.156 nmol/min/μg.

Human

E. coli

N-6*His

Q9UNP9 (M1-V301)

10450  [NCBI]

MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.