Betacellulin/BTC 蛋白, Mouse (HEK293)

Probetacellulin Protein, Mouse (HEK293), a member of the epidermal growth factor (EGF) family, induces differentiation of pancreatic β-cells and promotes regeneration of β-cells in experimental diabetes.

Betacellulin (BTC) 是表皮生长因子 (EGF) 家族的成员，可诱导胰腺 β 细胞分化并促进实验性糖尿病中 β 细胞的再生。BTC 刺激成纤维细胞和血管平滑肌细胞中的 DNA 合成。BTC 在调节胎儿胰腺内分泌前体细胞的生长和/或分化中发挥作用。研究发现 BTC 可将分泌淀粉酶的胰腺 AR42J 细胞转化为胰岛素生成细胞，并对人类未分化胰腺上皮细胞具有促有丝分裂作用^[1]。

The ED₅₀ is <0.08 ng/mL as measured by 3T3 cells.

Mouse

HEK293

Tag Free

Q05928 (D32-Y111)

12223  [NCBI]

DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFY

19-24 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Li L, et al. Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells to beta-cells in streptozotocin-treated mice. Am J Physiol Endocrinol Metab. 2003 Sep;285(3):E577-83.  [Content Brief]