QPCT 蛋白, Human (sf9, His)

QPCT Protein synthesizes pyroglutamyl peptides, favoring N-terminal glutaminyl residue over adjacent acidic and tryptophan residues. It catalyzes N-terminal pyroglutamate formation, particularly [Glu-3]-amyloid-beta in APP peptides. In lab settings, it aids pyroglutamate formation in truncated amyloid-beta peptides. QPCT Protein may contribute to N-terminal pyroglutamate formation in peptides linked to amyloid-related plaque development. QPCT Protein, Human (sf9, His) is the recombinant human-derived QPCT protein, expressed by Sf9 insect cells , with N-His labeled tag.

QPCT 蛋白负责焦谷氨酰肽的合成，对 N 端谷氨酰胺酰残基的相邻酸性和色氨酸残基表现出偏好，同时对第二个残基以外的链长度影响最小。它还催化 N 末端焦谷氨酸的形成。在实验室环境中，它促进 APP 淀粉样蛋白-β 肽（特别是 [Glu-3]-淀粉样蛋白-β）截短形式的焦谷氨酸形成。QPCT 蛋白也可能在与淀粉样蛋白相关斑块形成相关的各种肽的 N 末端焦谷氨酸形成中发挥作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

Q16769-1 (A33-L361)

25797  [NCBI]

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.