Ribonuclease R (rnr) 蛋白, E.coli (His)

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease crucial for structured RNA maturation, degrading rRNAs, tRNAs, and SsrA/tmRNA. It modulates ompA mRNA stability in the stationary phase, exhibiting processive activity and helicase function. RNase R, along with PNPase, ensures rRNA quality during steady-state growth and contributes to virulence gene expression in E. coli. Ribonuclease R (rnr) protein, E.coli (His) is the recombinant E. coli-derived Ribonuclease R, expressed by E. coli , with N-6*His labeled tag. The total length of Ribonuclease R (rnr) protein, E.coli (His) is 813 a.a., with molecular weight of ~90 kDa.

核糖核酸酶 R (RNase R) 是一种 3'-5' 核糖核酸外切酶，在结构化 RNA（包括 rRNA、tRNA 和 SsrA/tmRNA）的成熟中发挥着至关重要的作用。它通过在降解过程中释放 5'-核苷单磷酸发挥作用。此外，在稳定期，RNase R 参与转录后调节，特别是 ompA mRNA 稳定性的调节。它表现出加工活性，将 RNA 分子缩短为二核苷酸和三核苷酸。有趣的是，RNase R 还具有解旋酶活性，这与其 RNase 功能不同。饥饿期间，RNase 2 和 R（rnb 和 RNase R）有助于 rRNA 的降解。然而，在正常生长条件下，RNase R 与 PNPase 在 rRNA 的质量控制中发挥着更重要的作用。这些酶共同作用，确保 rRNA 在稳态生长过程中正常发挥作用和保持完整性。此外，RNase R 是肠侵袭性大肠杆菌菌株毒力基因表达所必需的。

Measured by its ability to digest RNA, it reacted at 37°C for 30 minutes under a certain reaction system, and was identified by agar-gel electrophoresis. The optimal reaction concentration measured under the above conditions was 120 μg/mL-240 μg/mL.

E.coli

E. coli

N-6*His

P21499 (M1-E813)

948692

MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAGVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE

Approximately 90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% TX-100, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.