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  3. ROBO4 蛋白, Mouse (453a.a, HEK293, His)

ROBO4 蛋白, Mouse (453a.a, HEK293, His)

目录号: HY-P78031
产品使用指南

ROBO4蛋白作为Slit蛋白(尤其是SLIT2)的受体,在血管生成和血管模式形成中发挥着关键作用。它介导 Slit 蛋白对原代内皮细胞迁移的抑制,并积极维持内皮屏障组织和功能。ROBO4 蛋白, Mouse (453a.a, HEK293, His) 是重组的 ROBO4 蛋白,由 HEK293 表达,带有 C-His 标签。ROBO4 蛋白, Mouse (453a.a, HEK293, His) 全长 453 个氨基酸,分子量为 60-70 kDa。

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描述

ROBO4 protein, as a receptor for Slit proteins (especially SLIT2), plays a key role in angiogenesis and blood vessel pattern formation. It mediates the inhibition of primary endothelial cell migration by Slit protein and actively maintains endothelial barrier organization and function. ROBO4 Protein, Mouse (453a.a, HEK293, His) is the recombinant mouse-derived ROBO4 protein, expressed by HEK293 , with C-His labeled tag. The total length of ROBO4 Protein, Mouse (453a.a, HEK293, His) is 453 a.a., with molecular weight of 60-70 kDa.

研究背景

ROBO4 蛋白作为 Slit 蛋白(尤其是 SLIT2)的受体,在血管生成和血管模式形成中发挥着至关重要的作用。该受体参与介导 Slit 蛋白对原代内皮细胞迁移的抑制。此外,ROBO4 积极参与维持内皮屏障的组织和功能。通过与 SLIT2 和 ENAH 的相互作用,ROBO4 有助于复杂的细胞过程,揭示其在血管发育和内皮细胞行为调节中的重要性。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q8C310 (L28-W480)

基因 ID

74144  [NCBI]

蛋白结构
N-term
ROBO4 (L28-W480)
Accession # Q8C310
His
C-term
同用名
ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
分子量

60-70 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ROBO4 Protein, Mouse (453a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ROBO4 Protein, Mouse (453a.a, HEK293, His)
目录号:
HY-P78031
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