ROR2 蛋白, Human (HEK293, His)

The ROR2 protein is a tyrosine protein kinase receptor that plays a critical role in early chondrocyte formation and is essential for cartilage and growth plate development. Phosphorylated YWHAB induces osteogenesis and bone formation. ROR2 Protein, Human (HEK293, His) is the recombinant human-derived ROR2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ROR2 Protein, Human (HEK293, His) is 370 a.a., with molecular weight of 50-60 kDa.

ROR2 蛋白是一种酪氨酸蛋白激酶受体，在软骨细胞形成的早期阶段发挥着关键作用，被认为对于软骨和生长板的发育至关重要。其YWHAB磷酸化不仅能诱导成骨，还能促进骨形成。尽管在体外表现出最小的酪氨酸激酶活性，ROR2 的复杂作用还延伸到潜在地充当 Wnt 配体 WNT5A 的受体。这种相互作用可能会导致抑制 WNT3A 介导的信号传导的有趣结果。ROR2 的多方面功能凸显了其在协调软骨形成、成骨和 Wnt 信号通路复杂调节等复杂过程中的重要性。

Measured in a cell inhibition assay by using A375 cells. The ED₅₀ this effect is 1.493 μg/mL, corresponding to a specific activity is 669.7924 units/mg.

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  Measured in a cell inhibition assay by using A375 cells. The ED₅₀ this effect is 1.493 μg/mL, corresponding to a specific activity is 669.7924 units/mg.

Human

HEK293

C-His

Q01974 (E34-G403)

4920  [NCBI]

EVEVLDPNDPLGPLDGQDGPIPTLKGYFLNFLEPVNNITIVQGQTAILHCKVAGNPPPNVRWLKNDAPVVQEPRRIIIRKTEYGSRLRIQDLDTTDTGYYQCVATNGMKTITATGVLFVRLGPTHSPNHNFQDDYHEDGFCQPYRGIACARFIGNRTIYVDSLQMQGEIENRITAAFTMIGTSTHLSDQCSQFAIPSFCHFVFPLCDARSRTPKPRELCRDECEVLESDLCRQEYTIARSNPLILMRLQLPKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGMDYRGTASTTKSGHQCQPWALQHPHSHHLSSTDFPELGGGHAYCRNPGGQMEGPWCFTQNKNVRMELCDVPSCSPRDSSKMG

50-60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.