RORa 蛋白, Human (Sf9, His)

The RORa protein is a nuclear receptor that is critical for a variety of physiological processes including development, immunity, circadian rhythms, and metabolic pathways. RORa operates as a monomer, binding DNA to RORE and exhibiting intrinsic transcriptional activity. RORa Protein, Human (Sf9, His) is the recombinant human-derived RORa protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of RORa Protein, Human (Sf9, His) is 253 a.a., .

RORa 蛋白是一种核受体，在多种生理过程中发挥关键调节作用，包括胚胎发育、细胞分化、免疫、昼夜节律以及脂质、类固醇、异生物质和葡萄糖的代谢。作为单体，RORa 将 DNA 与 ROR 响应元件 (RORE) 结合，并表现出内在的转录活性。氧甾醇等天然配体充当激动剂或反向激动剂，调节其转录活性。RORa 将不同的辅因子组合招募到目标基因调控区域，根据组织、时间和启动子环境调节其表达。其调节影响延伸至参与光感受器和骨骼肌发育、小脑发育、颗粒细胞增殖、钙介导的信号转导和时钟基因的昼夜节律表达的基因。在肝脏中，RORa 与 RORC 一起充当脂质、类固醇和外源代谢基因的正向或负向调节剂。它还在肝脏葡萄糖代谢和脂肪细胞分化调节中发挥作用。此外，RORa 参与 CD4(+) T 辅助细胞谱系分化为 T(H)17 细胞，抑制 NF-κ-B 信号传导，并在缺氧信号传导中与 HIF1A 相互作用。RORa 与各种辅助因子和调控元件的多方面相互作用强调了其在协调复杂生物过程中的多功能作用。

Human

Sf9 insect cells

N-8*His

P35398 (A271-G523)

6095

31.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.