SARS-CoV-2 3C-like Proteinase (His)

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus^[1].

3C蛋白酶因其在不同小核糖核酸病毒之间的结构相似性以及与宿主蛋白的最小序列相似性而被认为是有效的靶标；因此，针对 3C 蛋白酶的有效抑制剂作为抗病毒药物的开发正在进行中。鸭甲型肝炎病毒 (DHAV) 属于 Picornavidea 家族，是家禽业的主要威胁^[1]。

Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl- KTSAVLQSGFRKME-Edans (Ssynthetic). The specific activity is >13000 pmol/min/mg.

Virus

E. coli

N-6*His

YP_009725301.1 (S1-Q306)

43740578  [NCBI]

HHHHHHSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

Approximately 33.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filter solution of PBS, 1 mM EDTA, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Sun D, et al. Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization. Virol J. 2019 Apr 29;16(1):54.  [Content Brief]