SARS Plpro/papain-like protease 蛋白 (His-Avi)

The multifunctional SARS Plpro/papain-like protease protein utilizes essential proteases for multi-protein cleavage to strategically coordinate viral RNA transcription and replication. It blocks host translation by interacting with the 40S ribosomal subunit, causing endonucleolytic cleavage near the 5'UTR of host mRNAs, thereby targeting them for degradation. SARS Plpro/papain-like protease Protein (His-Avi) is the recombinant Virus-derived SARS Plpro/papain-like protease protein, expressed by E. coli , with N-His, N-Avi labeled tag. The total length of SARS Plpro/papain-like protease Protein (His-Avi) is 314 a.a., with molecular weight of ~38.6 kDa.

SARS Plpro/木瓜蛋白酶样蛋白酶蛋白在病毒 RNA 转录和复制的复杂环境中作为多功能协调器出现，利用关键的蛋白酶进行多蛋白切割。它通过与 40S 核糖体亚基相互作用来阻碍宿主翻译，随后形成的 nsp1-40S 核糖体复合物在宿主 mRNA 的 5'UTR 附近诱导核酸内切切割，从而有效地靶向它们进行降解。值得注意的是，病毒 mRNA 部署了针对这种降解的防御机制，因为由于 5' 端前导序列的保护性存在，它们不易受到 nsp1 介导的核酸内切 RNA 切割的影响。通过抑制宿主基因表达，nsp1 促进受感染细胞内病毒基因的有效表达，同时逃避宿主免疫反应。此外，该蛋白可能通过结合和取代宿主 NUP93 来破坏核孔功能。SARS Plpro 的多方面功能凸显了其在塑造病毒生命周期和逃避宿主防御方面的关键作用。

Virus

E. coli

N-His;N-Avi

P0C6U8 (E1-I314)

/

Approximately 38.6 kDa

Greater than 95% as determined by Tris-Bis PAGE

<1 EU/μg, determined by LAL method.