Serpin G1 蛋白, Rat (HEK293, His)

Serpin G1 protein binds complement and inhibits serine-type endopeptidases. It is located in the extracellular space and is involved in aging. Serpin G1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin G1 protein, expressed by HEK293 , with C-His labeled tag.

Serpin G1 蛋白预计具有补体结合活性和丝氨酸型内肽酶抑制剂活性。它在衰老中发挥作用，位于细胞外空间。该蛋白已用于胶质母细胞瘤的研究，并可作为急性心肌梗死和急性坏死性胰腺炎的生物标志物。在人类中，该基因的直系同源物与多种疾病有关，包括 COVID-19、血管性水肿、脑梗塞、胰腺炎和中毒性休克综合征。该基因的人类直系同源物是 SERPING1，它是丝氨酸蛋白酶抑制蛋白家族 G 的成员。丝氨酸蛋白酶抑制蛋白 G1 蛋白在肝脏、肺和其他七个组织中表现出偏向表达。

Measured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC₅₀ value is 6.646 nM, as measured under the described conditions.

Rat

HEK293

C-His

Q6P734/NP_954524.1 (D23-A504)

295703  [NCBI]

DSEVTSHSSQDPLVVQEGSRDSVPERDGSRSPIEHTGQSSTWPTTSGSTKISNDTMDQVANESFIQHVQPAAQLPEDSPSQSPVNSSSPPSTASAPPTQAPTEPLCPEPLAWCSDSDRDSSEATLSEALTDFSVKLYHAFSATKKAETNMAFSPFSIASLLTQVLLGAGDSTKSNLEDILSYPKDFACVHQTLKAFSSKGVTSVSQIFHSPDLAIRDTYVNASLSLYGSSPRVLGPDGDANLKLINTWVAENTNHKINELLDSLPSDTRLVLLNAVYLSAKWKKTFEQKKMMASFLYKNSMIKVPMLSSKKYPLALFNDQTLKAKVGQLQLSHNLSFVIMVPQSPTHQLEDMEKALNPTVFKAILKKLELSKFQPTYVMMPRIKVKSSQDMLSIMEKLEFFDFTYDLNLCGLTEDPDLQVSSMKHETVLELTETGVEAAAASTISVARNLLIFEVQQPFLFLLWDQRHKFPVFMGRVYDPRA

Approximately 70-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE. The protein migrates as a 70-95 kDa band under reducing SDS-PAGE due to glycosylation.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.