SIRT1 蛋白, Human (His)

SIRT1 is an NAD-dependent protein deacetylase that integrates multiple cellular functions—cell cycle progression, DNA damage response, metabolism, apoptosis, and autophagy—by regulating the acetylation of various target proteins.SIRT1 responds to changes in the NAD(+)/NADH ratio caused by factors such as glucose deprivation, affecting chromatin remodeling through histone deacetylation, resulting in transcriptional regulation. SIRT1 Protein, Human (His) is the recombinant human-derived SIRT1 protein, expressed by E.coli , with N-6*His labeled tag.

SIRT1 是一种 NAD 依赖性蛋白脱乙酰酶，通过调节众多靶蛋白的乙酰化状态，协调各种细胞功能的整合，包括细胞周期进程、对 DNA 损伤的反应、代谢、细胞凋亡和自噬。SIRT1 作为 NAD(+)/NADH 比率的传感器，对葡萄糖剥夺和热量限制等因素引起的细胞能量变化做出反应。其广泛的影响包括通过组蛋白脱乙酰化进行染色质重塑，最终导致转录调控。SIRT1 参与多种相互作用，使转录因子、共调节子和组蛋白去乙酰化，从而对靶基因表达发挥正向和负向调节作用。此外，SIRT1 还涉及 DNA 损伤反应、自噬、昼夜节律调节和代谢稳态等关键过程。其多方面的作用延伸到各种细胞途径的调节和基因组完整性的维持，突出了其在细胞生理学和应激反应中的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q96EB6 (A2-S747)

23411  [NCBI]

ADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS

Approximately 90 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.