1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. SVMP 蛋白, Crotalus adamanteus (His)

SVMP 蛋白, Crotalus adamanteus (His)

目录号: HY-P71455
COA 产品使用指南

SVMP 蛋白虽然缺乏显着的出血活性,但通过对其反应位点环进行有限的蛋白水解来灭活丝氨酸蛋白酶抑制剂而发挥作用。SVMP 蛋白, Crotalus adamanteus (His) 是重组的 SVMP 蛋白,由 E. coli 表达,带有 N-6*His 标签。SVMP 蛋白, Crotalus adamanteus (His) 全长 203 个氨基酸,分子量约为 ~27.1 kDa。

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规格 价格 是否有货 数量
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5 μg ¥930 In-stock
10 μg ¥1480 In-stock
20 μg ¥2350 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

SVMP protein, while lacking significant hemorrhagic activity, functions by inactivating serpins through limited proteolysis of their reactive-site loops. SVMP Protein, Crotalus adamanteus (His) is the recombinant SVMP protein, expressed by E. coli , with N-6*His labeled tag. The total length of SVMP Protein, Crotalus adamanteus (His) is 203 a.a., with molecular weight of ~27.1 kDa.

研究背景

SVMP蛋白没有表现出明显的出血活性;然而,它的作用是通过对丝氨酸蛋白酶抑制剂的反应位点环进行有限的蛋白水解来使其失活。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

P34179 (1Q-203P)

基因 ID

/

蛋白结构
N-term
6*His
SVMP (1Q-203P)
Accession # P34179
C-term
同用名
Snake venom metalloproteinase adamalysin-2; SVMP; EC 3.4.24.46; Adamalysin II; Proteinase II
氨基酸序列

QQNLPQRYIELVVVADRRVFMKYNSDLNIIRTRVHEIVNIINGFYRSLNIDVSLVNLEIWSGQDPLTIQSSSSNTLNSEGLWREKVLLNKKKKDNAQLLTAIEFKCETLGKAYLNSMCNPRSSVGIVKDHSPINLLVAVTMAHELGHNLGMEHDGKDCLRGASLCIMRPGLTPGRSYEFSDDSMGYYQKFLNQYKPQCILNKP

分子量

Approximately 27.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

SVMP Protein, Crotalus adamanteus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SVMP Protein, Crotalus adamanteus (His)
目录号:
HY-P71455
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