1. 重组蛋白
  2. Immune Checkpoint Proteins
  3. Inhibitory Checkpoint Molecules
  4. TIGIT Protein
  5. TIGIT 蛋白, Cynomolgus (HEK293, C-hFc)

TIGIT 蛋白, Cynomolgus (HEK293, C-hFc)

目录号: HY-P71007
产品使用指南

含有免疫球蛋白样结构域的蛋白是一类可能参与蛋白-蛋白和蛋白-配体相互作用的蛋白,因此参与了下调 T 细胞活化、心脏和神经元发育等生物学过程。TIGIT Protein, Cynomolgus (HEK293, C-hFc) 是重组的 TIGIT 蛋白,由 HEK293 表达,带有 C-hFc 标签。TIGIT Protein, Cynomolgus (HEK293, C-hFc) 全长 121 个氨基酸,分子量为 45-55 kDa。

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描述

Ig-like domain-containing protein is a class of proteins that may be involved in protein–protein and protein–ligand interactions, therefore being involved in biological processes such as down-regulation of T cell activation or cardiac and neuronal development. TIGIT Protein, Cynomolgus (HEK293, C-hFc) is the recombinant cynomolgus-derived TIGIT protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIGIT Protein, Cynomolgus (HEK293, C-hFc) is 121 a.a., with molecular weight of 45-55 kDa.

研究背景

含有免疫球蛋白样结构域的蛋白质是一类可能参与蛋白质-蛋白质和蛋白质-配体相互作用的蛋白质。一些包含免疫球蛋白样结构域的蛋白可与膜免疫球蛋白信号受体结合,通过与免疫球蛋白竞争结合下调 T 细胞活化,包含免疫球蛋白样结构域的蛋白如神经调节蛋白可通过 IgL 样结构域与肝素结合,促进心脏和神经元的发育[1][2]

种属

Cynomolgus

表达系统

HEK293

标签

C-hFc

蛋白编号

G7NXM4 (M89-P209)

基因 ID

102121588

蛋白结构
N-term
TIGIT (M89-P209)
Accession # G7NXM4
hFc
C-term
同用名
T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9
氨基酸序列

MMTGTIETTGNISAKKGGSVILQCHLSSTMAQVTQVNWEQHDHSLLAIRNAELGWHIYPAFKDRVAPGPGLGLTLQSLTMNDTGEYFCTYHTYPDGTYRGRIFLEVLESSVAEHSARFQIP

分子量

45-55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TIGIT Protein, Cynomolgus (HEK293, C-hFc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIGIT Protein, Cynomolgus (HEK293, C-hFc)
目录号:
HY-P71007
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