TM4SF2/TSPAN7 蛋白, Human (HEK293, His)

TM4SF2/TSPAN7 Protein is a member of the tetraspanin family. It mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN7 promotes apoptosis and inhibits tumor growth and cell cycle progression in BCa via the regulation of multiple key components of the PTEN/PI3K/AKT pathway. During cell differentiation, TSPAN7 emerges as a master regulator of morphological changes through cytoskeletal remodeling. TM4SF2/TSPAN7 Protein, Human (HEK293, His) is the recombinant human-derived TM4SF2/TSPAN7 protein, expressed by HEK293 , with C-His labeled tag. The total length of TM4SF2/TSPAN7 Protein, Human (HEK293, His) is 101 a.a., with molecular weight of 24-30 kDa.

四跨膜蛋白-7 (TSPAN7) 是四跨膜蛋白家族的成员。它介导信号转导事件，在细胞发育、激活、生长和运动的调节中发挥作用。值得注意的是，这种编码的细胞表面糖蛋白已被证明在控制神经突生长中发挥着至关重要的作用。TSPAN7 的过度表达会激活 Bax、裂解 caspase-3 和 PTEN，并通过 PTEN/PI3K/AKT 途径抑制 BCa 细胞生长。TSPAN7 的低表达与对氧化应激的反应、MAPK 的调节、细胞群增殖、对肿瘤坏死因子的反应、细胞迁移的调节、程序性细胞死亡和血管生成的负调节相关。此外，TSPAN7 通过调节 Src、Pyk2 和微管信号，在破骨细胞骨吸收功能所需的细胞骨架组织中发挥重要作用^[1][2]。

When Recombinant Human TSPAN7 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Rabbit Anti-TSPAN7 Antibody. The ED₅₀ for this effect is 111.6 ng/mL.

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  When Recombinant Human TSPAN7 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Rabbit Anti-TSPAN7 Antibody. The ED₅₀ for this effect is 111.6 ng/mL.

Human

HEK293

C-His

AAH18036.1 (R113-M213)

7102  [NCBI]

RHEIKDTFLRTYTDAMQTYNGNDERSRAVDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFMETNM

Approximately 21-30 kDa due to glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.