TPST1 蛋白, Human (HEK293, His)

TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293 , with N-His labeled tag. ,

酪氨酰蛋白磺基转移酶 1 (TPST1) 是一种关键酶，利用 3'-磷酸腺苷酸硫酸盐 (PAPS) 作为共底物，催化多肽酸性基序内酪氨酸残基的 O-硫酸化。该酶促过程涉及将硫酸基转移至酪氨酸残基，从而调节具有酸性基序的蛋白质的结构和功能。TPST1 介导的酪氨酸硫酸化对于参与各种生物过程（包括细胞信号传导和受体激活）的蛋白质翻译后修饰至关重要。通过参与酪氨酸残基的硫酸化，TPST1 有助于蛋白质修饰的多样性，影响细胞途径中硫酸化蛋白质的相互作用和活性。这种酶在酪氨酸硫酸化中的作用强调了它在调节蛋白质功能和细胞信号事件中的重要性（改编自所提供的通道）。

Human

HEK293

N-His

O60507 (Q26-E370)

8460  [NCBI]

QHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE

Approximately 43-57 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.