TRAIL R1/TNFRSF10A 蛋白, Human (216a.a, HEK293, His)

TRAIL R1/TNFRSF10A protein is a receptor for TNFSF10/TRAIL, which recruits caspase-8 through FADD to initiate cell apoptosis and form a death-inducing signaling complex (DISC). It activates NF-kappa-B and interacts with TRADD and RIPK1 in the monomeric state. TRAIL R1/TNFRSF10A Protein, Human (216a.a, HEK293, His) is the recombinant human-derived TRAIL R1/TNFRSF10A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TRAIL R1/TNFRSF10A Protein, Human (216a.a, HEK293, His) is 216 a.a., with molecular weight of 19-30 kDa.

TRAIL R1/TNFRSF10A 蛋白充当细胞毒性配体 TNFSF10/TRAIL 的受体。激活后，接头分子 FADD 将 caspase-8 募集至受体，形成死亡诱导信号复合物 (DISC)，导致 caspase-8 蛋白水解激活并启动随后的 caspase 级联，介导细胞凋亡。此外，TRAIL R1/TNFRSF10A 还可促进 NF-kappa-B 的激活。在单体状态下，它可以与 TRADD 和 RIPK1 相互作用。此外，TRAIL R1/TNFRSF10A与TNFRSF10B形成同源寡聚物和异源寡聚物，并且三个TRAIL R1分子与TNFSF10同源三聚体相互作用。该受体还与 ARAP1 和 ZDHHC3 相互作用，进一步凸显其参与复杂的信号网络。在没有刺激的情况下，TRAIL R1/TNFRSF10A 与 BIRC2、DDX3X 和 GSK3B 相互作用，并且这种相互作用在受体刺激时增强，并伴随着 DDX3X 和 BIRC2 的裂解。这些复杂的相互作用强调了 TRAIL R1/TNFRSF10A 在细胞凋亡和信号传导途径中的多方面作用。

Human

HEK293

C-6*His

O00220 (A24-N239)

8797  [NCBI]

ASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHN

19-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.