1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UB2E3 蛋白, Human (Sf9, His, Strep)

UB2E3 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701490
产品使用指南

UB2E3 蛋白是泛素-蛋白酶体系统的关键成分。作为一种 E2 泛素结合酶,它在泛素与靶蛋白的附着中发挥着至关重要的作用。在体外,UB2E3 表现出多种催化能力,促进“Lys-11”、“Lys-48”和“Lys-63”连接的多泛素化,表明其参与多种细胞过程。UB2E3 蛋白, Human (Sf9, His, Strep) 是重组的 UB2E3 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UB2E3 蛋白, Human (Sf9, His, Strep) 全长 206 个氨基酸。

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描述

UB2E3 protein is a key ubiquitin-proteasome system component. As an E2 ubiquitin-conjugating enzyme, it plays a crucial role in the attachment of ubiquitin to target proteins. In vitro, UB2E3 exhibits diverse catalytic abilities, promoting polyubiquitination of “Lys-11,” “Lys-48,” and “Lys-63” linkages, suggesting its involvement in diverse cellular processes. UB2E3 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UB2E3 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UB2E3 Protein, Human (Sf9, His, Strep) is 206 a.a., .

研究背景

UB2E3 是泛素-蛋白酶体系统的关键组成部分,作为 E2 泛素结合酶,在泛素与靶蛋白的附着中发挥着至关重要的作用。在体外,UB2E3 表现出多功能的催化能力,促进“Lys-11”、“Lys-48”和“Lys-63”连接的多泛素化,反映了其参与多种细胞过程。值得注意的是,UB2E3 参与肾细胞跨上皮钠转运的调节,表明其在离子稳态中的重要性。此外,其在细胞生长停滞中的潜在作用强调了 UB2E3 对细胞调节机制的多方面贡献,强调了其在维持细胞稳态和功能中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

Q969T4 (S2-T207)

基因 ID

10477

蛋白结构
N-term
8*His-Strep
UB2E3 (S2-T207)
Accession # Q969T4
C-term
同用名
UBE2E3; Ubiquitin-conjugating enzyme E2 E3; E2 ubiquitin-conjugating enzyme E3; UbcH9; Ubiquitin carrier protein E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UB2E3 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UB2E3 Protein, Human (Sf9, His, Strep)
目录号:
HY-P701490
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