UBA1 蛋白, Human (sf9, His-GST)

The UBA1 protein initiates ubiquitin conjugation, marking proteins for degradation in the ubiquitin-proteasome system. UBA1 Protein, Human (sf9, His-GST) is the recombinant human-derived UBA1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of UBA1 Protein, Human (sf9, His-GST) is 1057 a.a., with molecular weight of ~130 kDa.

UBA1 蛋白在细胞过程中发挥着关键作用，因为它催化泛素结合的初始步骤，标记细胞蛋白通过泛素-蛋白酶体系统降解，如多项研究所述。这种酶活性涉及通过最初用 ATP 对其 C 末端甘氨酸残基进行腺苷酸化来激活泛素。随后，UBA1 将这种激活的泛素连接到 E1 中半胱氨酸残基的侧链，形成泛素-E1 硫酯并释放游离 AMP。UBA1 不可或缺的功能延伸到关键的细胞过程，包括辐射诱导灶的形成、及时的 DNA 修复以及对复制应激的反应。此外，UBA1 在促进 TP53BP1 和 BRCA1 在 DNA 损伤位点的募集方面发挥着作用，强调了其在协调 DNA 修复途径和维持基因组完整性方面的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

P22314 (S2-R1058)

7317  [NCBI]

Approximately 130 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 10% Glycerol, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.