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  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. ACSS1 蛋白, Human (P.pastoris, His)

ACSS1 蛋白, Human (P.pastoris, His)

目录号: HY-P71851
产品使用指南

ACSS1 蛋白, Human (P.pastoris, His) 是一种线粒体基质酶,在心脏、骨骼肌和棕色脂肪组织中表达。ACSS1 蛋白, Human 参与醋酸盐介导的表观遗传调控。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

ACSS1 Protein, Human (P.pastoris, His) is a mitochondrial matrix enzyme that is expressed in heart, skeletal muscle and brown adipose tissue. ACSS1 Protein, Human is involved in acetate-mediated epigenetic regulation.

研究背景

酰基辅酶A短链合成酶(ACSS1、ACSS2、ACSS3)是唯一已知的能够将游离乙酸盐转化为乙酰辅酶A的哺乳动物酶。ACSS1 和 ACSS2 主要使用乙酸盐作为底物,而 ACSS3 优先使用丙酸盐。这些酶的活性部分受到特定赖氨酸位点的乙酰化和脱乙酰化的调节。乙酰化会使 ACSS1 和 ACSS2 失活,而去乙酰化则会重新激活它们。ACSS1 是一种线粒体基质酶,在心脏、骨骼肌和棕色脂肪组织中强烈表达。该亚型主要生成乙酰辅酶 A,乙酰辅酶A 将进入柠檬酸循环,通过氧化为 CO2[1] 获取能量。

种属

Human

表达系统

P. pastoris

标签

N-His

蛋白编号

Q9NUB1-1 (A38-K689)

基因 ID
蛋白结构
N-term
His
ACSS1 (A38-K689)
Accession # Q9NUB1-1
C-term
同用名
ACAS2L; Acetate CoA ligase 2; Acetate--CoA ligase 2
氨基酸序列

ASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK

分子量

Approximately 73.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

ACSS1 Protein, Human (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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