AGO1 蛋白, Human (sf9, His)

The AGO1 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs) and inhibiting the translation of complementary mRNAs.Despite lacking endonuclease activity, AGO1 does not cleave target mRNA.AGO1 Protein, Human (sf9, His) is the recombinant human-derived AGO1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

AGO1 是 RNA 介导的基因沉默 (RNAi) 的关键成分，通过与 microRNA (miRNA) 或短干扰 RNA (siRNA) 等短 RNA 结合发挥作用，从而通过互补碱基配对协调 mRNA 翻译的抑制。值得注意的是，AGO1 缺乏内切核酸酶活性，与其对应物不同，并且不参与靶 mRNA 的切割。除了转录后基因沉默之外，AGO1 在转录基因沉默 (TGS) 中也发挥着重要作用，作用于与结合的短反基因 RNA (agRNA) 具有互补性的启动子区域。其广泛的分子相互作用网络涉及 DDB1、DDX5、DDX6、DHX30、DHX36、DDX47、DICER1、AGO2、ELAVL1、HNRNPF、IGF2BP1、ILF3、IMP8、MATR3、MOV10、PABPC1、PRMT5、RBM4、SART3、TNRC6B 等关键参与者、UPF1、YBX1、LIMD1、WTIP、AJUBA、APOBEC3F、APOBEC3G 和 APOBEC3H。此外，AGO1 与多核糖体和信使核糖核蛋白 (mNRP) 的关联强调了其在各个阶段的基因表达动态调节中的不可或缺的作用。

Human

Sf9 insect cells

N-His

Q9UL18 (M1-A857)

26523  [NCBI]

Approximately 99.5 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Gly, 0.5 PMSF, 0.5 mM EDTA, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.