Animal-Free IL-13 蛋白, Mouse (His)

IL-13 protein is a cytokine involved in allergic inflammation, immune response to parasites, and B cell proliferation. Animal-Free IL-13 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-13 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-13 Protein, Mouse (His) is 110 a.a., with molecular weight of ~13.06 kDa.

IL-13 蛋白在过敏性炎症和寄生虫感染的免疫反应中发挥着关键作用。它与 IL2 协同调节干扰素-γ 合成、刺激 B 细胞增殖以及激活嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞。除了其免疫功能外，IL-13 通过调节跨膜和可溶形式的 IL-1 受体样 1/IL1RL1 的产生，在控制 IL33 活性方面发挥着至关重要的作用。它展示了拮抗 Th1 驱动的促炎性免疫反应并下调各种促炎细胞因子（包括 IL1、IL6、IL10、IL12 和 TNF-α）合成的能力，部分通过抑制 NF-kappa-B 实现。IL-13 不仅作用于造血细胞，还作用于内皮细胞等非造血细胞，诱导血管细胞粘附蛋白 1/VCAM1，这对于嗜酸性粒细胞的募集至关重要。其生物学效应是通过包含IL4R链和IL13RA1链的受体介导的，激活JAK1和TYK2，最终导致STAT6的激活。除了 IL13RA1 之外，另一种受体 IL13RA2 可作为 IL-13 的高亲和力诱饵，介导细胞外 IL-13 的内化和消耗。IL-13 直接与 IL13RA2 相互作用，进一步说明其调节机制的复杂性。

Measure by its ability to induce TF-1 cells proliferation.The ED₅₀ for this effect is<10 ng/mL.The specific activity of recombinant mouse IL-13 is >2.5x10⁵ IU/mg

Mouse

E. coli

C-His

P20109 (P22-F131)

16163  [NCBI]

MPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF

Approximately 13.06 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.