2. 重组蛋白
  3. Cytokines and Growth Factors CD Antigens
  4. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  5. TNF Superfamily Ligands CD30L/CD153 CD30L/CD153
  6. CD30L/CD153
  7. CD30 Ligand/TNFSF8 蛋白, Human (HEK293, N-His)

CD30 Ligand/TNFSF8 蛋白, Human (HEK293, N-His)

目录号: HY-P700434
产品使用指南

CD30 配体/TNFSF8 蛋白是一种细胞因子，可特异性结合 TNFRSF8/CD30，并充当 T 细胞增殖的有效诱导剂。作为同源三聚体，该配体在调节 T 细胞活化和生长中发挥着至关重要的作用，有助于免疫反应的动态协调。CD30 Ligand/TNFSF8 蛋白, Human (HEK293, N-His) 是重组的 CD30 配体/TNFSF8 蛋白，由 HEK293 表达，带有 N-6*His 标签。CD30 Ligand/TNFSF8 蛋白, Human (HEK293, N-His) 全长 172 个氨基酸，分子量为 21.8 kDa。

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描述

CD30 ligand/TNFSF8 protein is a cytokine that specifically binds to TNFRSF8/CD30 and acts as a potent inducer of T cell proliferation. As a homotrimer, this ligand plays a crucial role in regulating T cell activation and growth, contributing to the dynamic coordination of immune responses. CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is the recombinant human-derived CD30 Ligand/TNFSF8 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of CD30 Ligand/TNFSF8 Protein, Human (HEK293, N-His) is 172 a.a., with molecular weight of 21.8 kDa.
研究背景

CD30 配体/TNFSF8 蛋白是一种细胞因子，可特异性结合 TNFRSF8/CD30，充当 T 细胞增殖的有效诱导剂。作为同源三聚体，该配体在调节 T 细胞的激活和生长中发挥着至关重要的作用，有助于免疫反应的动态协调。CD30 配体与其受体 TNFRSF8/CD30 之间的相互作用凸显了其作为 T 细胞群体强劲扩张的分子触发因素的重要性。
种属

Human
表达系统

HEK293
标签

N-6*His
蛋白编号

P32971 (Q63-D234)
基因 ID

944  [NCBI]
蛋白结构
N-term
6*His
CD30L (Q63-D234)
Accession # P32971
C-term
同用名
Tumor necrosis factor ligand superfamily member 8; CD153; CD30L; CD30LG
氨基酸序列

QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD

分子量

21.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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