CD8 beta 蛋白, Human (HEK293, His)

CD8 beta is an important immune glycoprotein that serves as a coreceptor for MHC class I:peptide complexes, linking T cells to antigens presented by APCs. It interacts with TCR and MHC class I, recruits LCK kinase, and initiates multiple signaling pathways. CD8 beta Protein, Human (HEK293, His) is the recombinant human-derived CD8 beta protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD8 beta Protein, Human (HEK293, His) is 149 a.a., with molecular weight of 25-32 kDa.

CD8 β 是一种完整的膜糖蛋白，在免疫反应中发挥着关键作用，具有针对外部和内部威胁的多种功能。在 T 细胞中，其主要功能是作为 MHC I 类分子：肽复合物的辅助受体，其中 I 类肽呈递的抗原源自胞质蛋白。CD8 beta 同时与 T 细胞受体 (TCR) 和抗原呈递细胞 (APC) 呈递的 MHC I 类蛋白相互作用，将 Src 激酶 LCK 招募到 TCR-CD3 复合物附近。CD8B 链胞质尾部存在棕榈酰化位点，有利于 CD8 分配到质膜脂筏中，从而富集信号蛋白。一旦 LCK 被招募，它就会启动多种细胞内信号传导途径，磷酸化各种底物并导致淋巴因子的产生、运动、粘附和细胞毒性 T 淋巴细胞 (CTL) 的激活。CD8β 在 CD8+ T 细胞的胸腺选择中也发挥着关键作用。在细胞表面，它与 CD8A 形成二硫键连接的异二聚体，并与 CD3D 相互作用，将 TCR-CD3 与 CD8 偶联，还与 LCK 相互作用。

Human

HEK293

C-6*His

P10966-1 (L22-P170)

926  [NCBI]

LQQTPAYIKVQTNKMVMLSCEAKISLSNMRIYWLRQRQAPSSDSHHEFLALWDSAKGTIHGEEVEQEKIAVFRDASRFILNLTSVKPEDSGIYFCMIVGSPELTFGKGTQLSVVDFLPTTAQPTKKSTLKKRVCRLPRPETQKGPLCSP

25-32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.