CD8A-CD8B Heterodimer 蛋白, Mouse (HEK293, Flag-His)

The CD8A, a vital immune glycoprotein, acts as a coreceptor for MHC class I:peptide complexes, connecting T-cells to antigens presented by APCs. Its interaction with TCR and MHC class I recruits LCK kinase, initiating diverse signaling pathways. This mechanism enables CTLs to recognize and eliminate infected and tumor cells. In NK-cells, CD8A homodimers provide a survival mechanism for conjugation and lysis of target cells, contributing to lymphocyte survival and differentiation into memory CD8 T-cells. The heterodimer forms disulfide-linked complexes and homodimers, interacting with HLA-A/B2M and LCK in a zinc-dependent manner. CD8A-CD8B Heterodimer Protein, Mouse (HEK293, Flag-His) is a recombinant protein dimer complex containing mouse-derived CD8A-CD8B Heterodimer protein, expressed by HEK293, with C-His, C-Flag labeled tag. CD8A-CD8B Heterodimer Protein, Mouse (HEK293, Flag-His), has molecular weight of ~45.9 (23.7+22.2) kDa.

CD8A 是一种完整的膜糖蛋白，在免疫反应中发挥着关键作用，在应对外部和内部威胁时发挥多种功能。在 T 细胞中，它主要充当 MHC I 类分子：肽复合物的辅助受体，同时与 T 细胞受体 (TCR) 和抗原呈递细胞 (APC) 呈递的 MHC I 类蛋白相互作用。这种相互作用导致 Src 激酶 LCK 募集到 TCR-CD3 复合物附近。LCK 反过来启动多种细胞内信号传导途径，磷酸化各种底物，最终促进细胞毒性 T 淋巴细胞 (CTL) 的淋巴因子产生、运动、粘附和激活。这种机制使 CTL 能够识别并消除受感染的细胞和肿瘤细胞。在 NK 细胞中，细胞表面 CD8A 同二聚体的存在提供了一种生存机制，允许多个靶细胞的接合和裂解。CD8A 同二聚体分子还有助于活化淋巴细胞的存活和分化为记忆 CD8 T 细胞。CD8A 在细胞表面形成二硫键连接的复合物，并在各种细胞类型（包括 NK 细胞和外周血 T 淋巴细胞）中形成同二聚体。它以锌依赖性方式与 MHC I 类 HLA-A/B2M 二聚体和 LCK 相互作用。

Mouse

HEK293

C-His;C-Flag

P01731 (K28-Y196)&P10300 (L22-T175)

12525  [NCBI]&12526  [NCBI]

Approximately 45.9 (23.7+22.2) kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.