Collectin-11/CL-K1 蛋白, Human (HEK293, His)

Collectin-11/CL-K1 is an important lectin in innate immunity, apoptosis, and embryogenesis that recognizes sugars presenting high mannose oligosaccharides with at least one terminal α-1,2-linked mannose epitope protein. It also recognizes fucosylated glycans and lipopolysaccharides. Collectin-11/CL-K1 Protein, Human (HEK293, His) is the recombinant human-derived Collectin-11/CL-K1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Collectin-11/CL-K1 Protein, Human (HEK293, His) is 246 a.a., with molecular weight of 30-35 kDa.

Collectin-11/CL-K1 蛋白是一种凝集素，对于先天免疫、细胞凋亡和胚胎发生至关重要。这种钙依赖性凝集素可识别呈递高甘露糖寡糖的自身和非自身糖蛋白，该寡糖具有至少一个末端 α-1,2 连接的甘露糖表位，主要集中于聚糖的末端二糖。此外，它还鉴定了岩藻糖基化聚糖和脂多糖的子集。在先天免疫中，Collectin-11/CL-K1 结合微生物上的非自糖，通过招募 MAPS1 激活补体。在细胞凋亡过程中，该蛋白以不依赖于钙的方式结合凋亡细胞表面的 DNA，从而触发补体激活以响应这种结合。此外，Collectin-11/CL-K1 有助于发育，可能在神经嵴细胞和胚胎发生中其他细胞类型的迁移过程中充当指导信号。该蛋白通过二硫键形成同源三聚体并与 MASP1 相互作用，可能启动补体激活的凝集素途径。

Human

HEK293

C-6*His

Q9BWP8 (Q26-M271)

78989  [NCBI]

QPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQKGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEMDNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGGTLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTFNKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM

30-35 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.