CPLX3 蛋白, Human (HEK293, His)

CPLX3 protein is a complex protein that regulates synaptic vesicle fusion through the SNARE protein complex. CPLX3 Protein, Human (HEK293, His) is the recombinant human-derived CPLX3 protein, expressed by HEK293 , with N-His labeled tag. The total length of CPLX3 Protein, Human (HEK293, His) is 154 a.a., with molecular weight of 27-33 kDa.

CPLX3 是一种复合蛋白，复杂地调节 SNARE 蛋白复合物，从而在突触小泡融合的调节中发挥关键作用。其重要性延伸到成人视网膜突触超微结构的维持，强调其参与基本神经元过程。从功能上讲，CPLX3 通过调节突触小泡的可用性和促进神经递质胞吐作用，特别是在视网膜的感光带突触处，对突触传递产生积极影响。此外，CPLX3 通过抑制 Ca(2+) 囊泡的强直释放，有助于抑制强直感光活性和基线“噪音”，同时促进诱发的同步和异步 Ca(2+) 囊泡释放。其调节作用的分子基础在于其与 SNARE 核心复合物的相互作用，特别是与 SNAP25、VAMP2 和 STX1A 结合，强调了其在神经元通讯的复杂机制中的多方面参与。

Human

HEK293

N-6*His

Q8WVH0 (M1-K154)

594855  [NCBI]

MAFMVKTMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERATLRSHFRDKYRLPKNETDESQIQMAGGDVELPRELAKMIEEDTEEEEEKASVLGQLASLPGLNLGSLKDKAQATLGDLKQSAEK

Approximately 27-33 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.