1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA3
  6. EphA3 蛋白, Mouse (HEK293, Fc)

EphA3 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75740
产品使用指南

EphA3 蛋白是一种受体酪氨酸激酶,它与膜结合的肝配蛋白配体混杂结合以启动双向信号传导。已知 EphA3 的激活优先与 EFNA5 结合并调节细胞间粘附、细胞骨架组织和迁移。EphA3 蛋白, Mouse (HEK293, Fc) 是重组的 EphA3 蛋白,由 HEK293 表达,带有 C-hFc 标签。EphA3 蛋白, Mouse (HEK293, Fc) 全长 541 个氨基酸,分子量约为 ~96 kDa。

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描述

The EphA3 protein is a receptor tyrosine kinase that promiscuously binds to membrane-bound ephrin ligands to initiate bidirectional signaling. Activation of EphA3 is known to preferentially bind to EFNA5 and regulates cell-cell adhesion, cytoskeletal organization, and migration. EphA3 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EphA3 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of EphA3 Protein, Mouse (HEK293, Fc) is 541 a.a., with molecular weight of ~96 kDa.

研究背景

EphA3 蛋白是一种受体酪氨酸激酶,与相邻细胞上的膜结合肝配蛋白家族配体混杂结合,启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导,而肝配蛋白配体下游的途径称为反向信号传导。EphA3 与肝配蛋白 A 配体高度混杂,对 EFNA5 表现出优先结合亲和力。被 EFNA5 激活后,EphA3 在调节细胞间粘附、细胞骨架组织和细胞迁移中发挥关键作用。此外,EphA3 参与心脏细胞迁移和分化,可能通过 EFNA1 激活来调节发育过程中房室管和房间隔的形成。在视网膜顶盖绘图中,EphA3 参与神经元的引导。此外,EphA3 可以控制神经肌肉回路发育过程中运动和感觉轴突的分离,但不是引导。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q8BRB1 (M1-H541)

基因 ID
蛋白结构
N-term
EphA3 (M1-H541)
Accession # Q8BRB1
hFc
C-term
同用名
Ephrin type-A receptor 3; EPH-like kinase 4; hEK4; EPHA3; ETK; ETK1; HEK; TYRO4
分子量

Approximately 96 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EphA3 Protein, Mouse (HEK293, Fc)
目录号:
HY-P75740
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