HMGN3 蛋白, Human (His)

HMGN3 binds to nucleosomes, regulates chromatin structure, and affects chromatin-dependent processes such as transcription and DNA repair. It affects insulin and glucagon levels and regulates pancreatic gene expression related to insulin secretion. HMGN3 Protein, Human (His) is the recombinant human-derived HMGN3 protein, expressed by E. coli , with N-His labeled tag. The total length of HMGN3 Protein, Human (His) is 77 a.a., with molecular weight of ~17 kDa.

HMGN3 是一种能够与核小体结合的蛋白质，对染色质结构进行调节控制，从而影响各种染色质依赖性过程，包括转录、DNA 复制和 DNA 修复。它在胰岛素和胰高血糖素水平的调节中发挥作用，并参与胰岛素分泌相关胰腺基因的调节。HMGN3 特异性结合葡萄糖转运蛋白 SLC2A2 的启动子区域，招募 PDX1 等转录因子，从而影响葡萄糖稳态。此外，它还调节 SLC6A9 的表达，SLC6A9 是一种甘氨酸转运蛋白，对于维持中枢神经系统内突触连接处的甘氨酸浓度至关重要。除了其代谢功能外，HMGN3 还可能有助于眼睛发育和星形胶质细胞功能。此外，HMGN3 与甲状腺受体的配体结合域相互作用，需要甲状腺激素的存在才能发挥其活性，并与转录调节因子 SEHBP 相互作用，突出其在细胞和生理过程中的多方面作用。

Human

E. coli

N-10*His

Q15651-2 (M1-N77)

9324  [NCBI]

MPKRKSPENTEGKDGSKVTKQEPTRRSARLSAKPAPPKPEPKPRKTSAKKEPGAKISRGAKGKKEEKQEAGKEGTEN

Approximately 17 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.