HSP10/EPF 蛋白, Human (His)

The HSP10/EPF protein is a cochaperone that plays a role in mitochondrial protein import and assembly. HSP10/EPF Protein, Human (His) is the recombinant human-derived HSP10/EPF protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of HSP10/EPF Protein, Human (His) is 102 a.a., with molecular weight of ~13 KDa.

HSP10/EPF 蛋白作为辅助伴侣蛋白，积极参与线粒体蛋白输入和大分子组装。它与 Hsp60 合作，促进输入蛋白质的正确折叠，并且在线粒体基质的应激条件下，可以防止错误折叠，同时促进未折叠多肽的重折叠和正确组装。这些伴侣蛋白的操作单元包含大亚基Hsp60的七聚环，形成背靠背双环结构。在循环过程中，Hsp60 环复合物结合未折叠的底物蛋白，然后与 ATP 结合并与辅助伴侣蛋白 Hsp10 的两个七聚环缔合。这导致底物蛋白被隔离在 Hsp60 的内腔内，从而允许不受干扰的折叠。所有 Hsp60 亚基中的同步 ATP 水解导致伴侣蛋白环解离，释放 ADP 和折叠的底物蛋白。HSP10/EPF 蛋白形成以环状结构排列的同七聚体，并与 Hsp60 四聚体相互作用，形成对称的足球复合物。这些分子相互作用强调了 HSP10/EPF 在协调线粒体蛋白折叠和组装中的重要作用。

Human

E. coli

P61604 (M1-D102)

3336  [NCBI]

MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD

Approximately 13 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.