IFN-alpha 5/IFNA5 蛋白, Human (P.pastoris, His)

IFN-alpha 5 (IFNA5), belongs to type I interferon family, is produced by macrophages with antiviral activities^[1]. IFN-alpha 5/IFNA5 Protein, Human (P.pastoris, His) contains 168 a.a. (L22-E189), produced in P. pastoris yeast cells with a C-terminal His-tag.

干扰素-α5（IFNA5；IFN-α5）属于α/β干扰素（IFN）家族，由巨噬细胞产生，具有抗病毒活性。干扰素（IFN）最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素，其他两类分别为II型干扰素（IFN-γ）和III型干扰素（IFN-λ）^[1]。
干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。干扰素 α (IFNa) 在多种癌症中显示出显着的生物活性，特别是血液系统恶性肿瘤，如毛细胞白血病和慢性粒细胞白血病^[2]。
IFN-α 5 参与先天免疫，并且是与呼吸道合胞病毒 (RSV) 引起的急性病毒性细支气管炎 (AVB) 相关的基因之一，决定了对 RSV 细支气管炎的易感性^[3][4]。
流感病毒感染过程中，过度表达的干扰素-α（IFN-α）可能会导致炎症反应失控，造成病理损伤。而IFN-α 5主要表达于低致病性传染性支气管炎病毒(IBV)感染者的呼吸道上皮细胞^[5]。
至于IFN在动物模型中的广泛使用，人IFNA5蛋白的氨基酸序列与小鼠有很大差异（60.32%）

IFN-α subtype shows a diversity in human respiratory epithelial cells infected with influenza viruses, which induces the dominant expression of IFN-α5, IFN-α8, and IFN-α21^[5].

Human

P. pastoris

C-His

P01569/NP_002160.1 (L22-E189)

3442  [NCBI]

Approximately 21.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40.  [Content Brief]

  [2]. Raj NB, et al. Identification of a novel virus-responsive sequence in the promoter of murine interferon-alpha genes. J Biol Chem. 1991 Jun 15;266(17):11360-5.  [Content Brief]

  [3]. Hirankarn N, et al. Genetic association of interferon-alpha subtypes 1, 2 and 5 in systemic lupus erythematosus. Tissue Antigens. 2008 Dec;72(6):588-92.  [Content Brief]

  [4]. Janssen R, et al. Genetic susceptibility to respiratory syncytial virus bronchiolitis is predominantly associated with innate immune genes. J Infect Dis. 2007 Sep 15;196(6):826-34.  [Content Brief]

  [5]. Yang L, et al. Diversity of locally produced IFN-α subtypes in human nasopharyngeal epithelial cells and mouse lung tissues during influenza virus infection. Appl Microbiol Biotechnol. 2020 Jul;104(14):6351-6361.  [Content Brief]