IFN-gamma R2 蛋白, Human (CHO)

IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2 is the signal-transducing chain of the IFN-gamma receptor. IFN-gamma R2 forms the functional receptor with IFN-gamma R1. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate^[1]. Then, the downstream signaling components JAK1 and JAK2 are phosphorylated and activated, and STAT1 is phosphorylated. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)^[2]. IFN-gamma R2 Protein, Human (CHO) is a recombinant human IFN-gamma R2 (S28-Q247) without any tag, which is produced in CHO cells.

IFN-γ R2是IFN-γ受体的亚基之一，是IFN-γ的受体。IFN-γ R2。IFN-γ R2 在骨髓细胞中高表达，在 B 细胞中中度表达，在 T 细胞中低表达，并且表达受细胞分化或激活状态的调节^[2]。
IFN -γR2可以与IFN-γR1结合形成功能性受体。与 IFN-gamma 结合后，IFN-gamma R2 和 IFN-gamma R1 寡聚并转磷酸化^[1]。然后，JAK1 和 JAK2 被磷酸化并激活，形成 STAT1 的结合位点。STAT1 被招募到受体复合物并导致磷酸化。磷酸化的 STAT1 易位到细胞核，在那里调节 IFN 反应基因（例如 CD54）的表达。IFN-γ R2 对于针对分枝杆菌感染的先天免疫防御非常重要。IFN-γ R2 缺陷导致孟德尔对分枝杆菌疾病 (MSMD) 的易感性^[2]。
人 IFN-γ R2 由胞外结构域 (S28-Q247)、螺旋结构域 (V248- F268) 和胞质结构域 (L269-L337)。
IFN-γ R2 在抗菌和免疫反应中发挥着关键作用^[2]。

ED₅₀ < 0.1 μg/ml, measured in a cell cytotoxicity assay using HT-29 (HTB-38) cells in the presence of 1.0 ng/ml human IFN-gamma.

Human

CHO

Tag Free

P38484 (S28-Q247)

3460  [NCBI]

SQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYQVQFKYTDSKWFTADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNISCYETMADASTELQQ

38-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by gel clotting method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Castro F, et al. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018 May 4;9:847.  [Content Brief]

  [2]. Pestka S, et al. The interferon gamma (IFN-gamma) receptor: a paradigm for the multichain cytokine receptor. Cytokine Growth Factor Rev. 1997 Sep;8(3):189-206.  [Content Brief]

  [3]. van de Vosse E, et al. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database. Hum Mutat. 2017 Oct;38(10):1286-1296.  [Content Brief]