IGF2BP2 蛋白, Human (sf9, His-GST)

The IGF2BP2 protein is an RNA-binding factor that coordinates the recruitment of target transcripts into cytoplasmic mRNP, promoting mRNA transport and transient storage. It regulates the contact of target transcripts with the translation machinery and protects them from microRNA-mediated degradation. IGF2BP2 Protein, Human (sf9, His-GST) is the recombinant human-derived IGF2BP2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of IGF2BP2 Protein, Human (sf9, His-GST) is 599 a.a., with molecular weight of ~90 kDa.

IGF2BP2 蛋白充当 RNA 结合因子，协调靶转录物招募到细胞质蛋白-RNA 复合物 (mRNP) 中。mRNP 内转录本的这种“囚禁”有利于 mRNA 运输和瞬时存储，同时调节目标转录本遇到翻译装置的速率和位置。此外，IGF2BP2 保护这些转录物免受核酸内切酶攻击或 microRNA 介导的降解。由于偏爱含有 N6-甲基腺苷 (m6A) 的 mRNA，IGF2BP2 增强了它们的稳定性。它与胰岛素样生长因子 2 (IGF2) mRNA 的 5'-UTR 特异性结合，并选择性地与 β-肌动蛋白/ACTB 和 MYC 等转录物结合，通过与编码区不稳定决定簇 (CRD) 相互作用提高 MYC mRNA 稳定性。IGF2BP2 可以以 RNA 依赖性方式与 IGF2BP1 和 IGF2BP3 形成同寡聚体和异寡聚体，并与多种蛋白质相互作用，包括 HNRPD、IGF2BP1、ELAVL1、DHX9、HNRNPU、MATR3 和 PABPC1。它与 HOXB-AS3 肽的相互作用进一步增强了 MYC 稳定性。

Human

Sf9 insect cells

N-His;N-GST

Q9Y6M1-2 (M1-K599)

10644  [NCBI]

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 5 mM Reduced Glutathione, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.