LAG-3 蛋白, Cynomolgus (428a.a, HEK293, His)

LAG-3 Protein, expressed on antigen-activated T-cells, delivers inhibitory signals by binding to ligands like FGL1. LAG-3 Protein associates with CD3-TCR, directly inhibiting T-cell activation^[1][2]. LAG-3 Protein, Cynomolgus (428a.a, HEK293, His) is a recombinant protein dimer complex containing cynomolgus-derived LAG-3 protein, expressed by HEK293 , with N-8*His labeled tag. LAG-3 Protein, Cynomolgus (428a.a, HEK293, His), has molecular weight of 55-65 kDa.

LAG-3 蛋白作为抗原激活 T 细胞的抑制性受体，与 FGL1 等配体结合后传递抑制信号，是 LAG3 T 细胞抑制功能的主要贡献者^[1][2]。在与 T 细胞受体 (TCR) 接合后，LAG-3 蛋白与免疫突触中的 CD3-TCR 结合，并直接阻断 T 细胞激活。LAG-3 蛋白可能与 PDCD1/PD-1 协同作用，潜在地充当 PD-1 的辅助受体并抑制抗原特异性 T 细胞激活^[3]。该蛋白负向调节 CD8+ 和 CD4+ T 细胞的增殖、激活、效应功能和稳态。此外，LAG-3 蛋白在免疫耐受中发挥着关键作用，并且在调节性 T 细胞 (Treg) 子集上组成型表达，有助于其抑制功能^[4]。LAG-3 蛋白充当浆细胞样树突状细胞 (pDC) 激活的负调节因子，并表现出与 MHC II 类 (MHC-II) 的相互作用，但 MHC-II 结合的确切作用仍不清楚。LAG-3 可能作为抗原呈递细胞 (APC) 上 MHC II 类的配体，可能促进 APC 激活/成熟并驱动 Th1 免疫反应。

Cynomolgus

HEK293

N-8*His

XP_005570011.1 (P23-L450(P74))

102122272  [NCBI]

PQPGAEISVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAPAPGHPPVPGHRPAAPYSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRATVHLRDRALSCRLRLRVGQASMTASPPGSLRTSDWVILNCSFSRPDRPASVHWFRSRGQGRVPVQGSPHHHLAESFLFLPHVGPMDSGLWGCILTYRDGFNVSIMYNLTVLGLEPATPLTVYAGAGSRVELPCRLPPAVGTQSFLTAKWAPPGGGPDLLVAGDNGDFTLRLEDVSQAQAGTYICHIRLQGQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPASGQEHFVWSPLNTPSQRSFSGPWLEAQEAQLLSQPWQCQLHQGERLLGAAVYFTELSSPGAQRSGRAPGALRAGHL

55-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.