LAG-3 蛋白, Human (HEK293, His)

LAG-3 (lymphocyte activating gene 3) protein is an inhibitory receptor on antigen-activated T cells and is critical for immune regulation. LAG-3 binds to FGL1 and transmits inhibitory signals that negatively affect CD8(+) and CD4(+) T cell proliferation, activation, and effector functions. LAG-3 Protein, Human (HEK293, His) is the recombinant human-derived LAG-3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LAG-3 Protein, Human (HEK293, His) is 428 a.a., with molecular weight of 60-80 kDa.

LAG-3（淋巴细胞激活基因 3）蛋白是抗原激活 T 细胞上的一种抑制性受体，在免疫调节中发挥着至关重要的作用。LAG-3 与其主要配体 FGL1 结合后，会传递抑制信号，对 CD8(+) 和 CD4(+) T 细胞的增殖、激活、效应器功能和稳态进行负调节。LAG-3 与 PDCD1/PD-1 协同作用，可以抑制抗原特异性 T 细胞激活，特别是在 T 细胞受体 (TCR) 与免疫突触中的 CD3-TCR 结合后。除了在 T 细胞抑制中的作用外，LAG-3 在调节性 T 细胞 (Treg) 子集上组成型表达，有助于其抑制功能并介导免疫耐受。此外，LAG-3 负向调节浆细胞样树突状细胞 (pDC) 的激活，并且有趣的是，它与 MHC II 类 (MHC-II) 相互作用，可能充当抗原呈递细胞 (APC) 上 MHC-II 的配体和启动子APC 的激活/成熟，从而影响 Th1 免疫反应。

Human

HEK293

C-6*His

P18627 (L23-L450)

3902  [NCBI]

LQPGAEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHL

60-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 2 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.