RYK 蛋白, Human (HEK293, Fc)

RYK protein, together with FZD8, serves as a potential Wnt co-receptor for WNT1, WNT3, WNT3A and WNT5A, affecting neuronal differentiation, axon guidance and neurite growth. After WNT3 stimulation, RYK undergoes C-terminal cleavage and transfers intracellular products to the nucleus, which is critical for neuronal development. RYK Protein, Human (HEK293, Fc) is the recombinant human-derived RYK protein, expressed by HEK293 , with C-hFc labeled tag.

RYK 蛋白似乎与 FZD8 合作，作为 Wnt 蛋白（如 WNT1、WNT3、WNT3A 和 WNT5A）的潜在辅助受体。它涉及神经元分化、轴突引导、胼胝体建立和神经突生长等关键过程，强调了它在神经发育中的重要性。受到 WNT3 刺激后，受体在其跨膜区域发生 C 末端裂解，促进 C 末端细胞内产物从细胞质易位至细胞核。这种易位产物在介导神经元发育中发挥着至关重要的作用。RYK 在 Wnt 信号通路中的多方面参与强调了其作为协调神经分化和生长所必需的复杂细胞过程的关键参与者的潜力。进一步探索 RYK 的功能和调节机制可能会更深入地了解其对神经元发育的具体贡献。

Measured by its binding ability in a functional ELISA. Immobilized Human RYK, at 2μg/mL (100 μL/well) can bind Wnt3a. The KD for this effect is 5.675 nM.

Human

HEK293

C-hFc

P34925/NP_002949.2 (P26-R227)

6259  [NCBI]

PPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSEDEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNISVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNTSRTIYDPVHAAPTTSTR

Approximately 49.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.