Serpin B1 蛋白, Human (HEK293, His)

Serpin B1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Serpin B1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin B1 Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~44.0 kDa.

Serpin B1 蛋白是一种中性粒细胞丝氨酸蛋白酶抑制剂，在调节先天免疫反应、炎症和细胞稳态中发挥着至关重要的作用。Serpin B1 主要作用是保护细胞免受应激或感染期间细胞质中释放的蛋白酶的影响，并防止颗粒释放的强效酶造成的潜在损害。它有效调节多种中性粒细胞蛋白酶的活性，包括弹性蛋白酶、组织蛋白酶 G、蛋白酶-3、糜酶、胰凝乳蛋白酶和激肽释放酶-3。值得注意的是，Serpin B1 还可以作为 GZMH 的有效细胞内抑制剂，直接阻断其蛋白水解活性。在炎症过程中，丝氨酸蛋白酶抑制剂 B1 通过抑制炎症性半胱天冬酶（CASP1、CASP4 和 CASP5）的寡聚化和酶激活来限制其活性。此外，当分泌时，Serpin B1 通过其蛋白酶抑制功能促进 β 细胞增殖。该蛋白作为单体发挥作用，并与 CASP1、CASP4、CASP5、PRTN3 和 GZMH 相互作用，揭示了其在调节各种细胞过程以及与免疫和炎症途径中关键蛋白相互作用中的复杂作用。

Human

HEK293

C-6*His

P30740 (M1-P379)

1992  [NCBI]

MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNTAAQLSKTFHFNTVEEVHSRFQSLNADINKRGASYILKLANRLYGEKTYNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSSKADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEENFTADHPFLFFIRHNSSGSILFLGRFSSP

Approximately 44.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.