Serpin B3 蛋白, Human (HEK293, His)

The Serpin B3 protein exhibits versatility in cellular regulation and may act as a papain-like cysteine protease inhibitor to modulate immune responses against tumor cells. Its dual role extends to inhibiting UV-induced apoptosis by inhibiting c-Jun NH(2)-terminal kinase (JNK1) activity, suggesting that Serpin B3 is involved in the stress response. Serpin B3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin B3 Protein, Human (HEK293, His) is 390 a.a., with molecular weight of 41-50 kDa.

Serpin B3 蛋白作为细胞调节的多功能参与者而出现，有可能作为木瓜蛋白酶样半胱氨酸蛋白酶抑制剂来调节宿主针对肿瘤细胞的免疫反应。其双重作用延伸至抑制紫外线诱导的细胞凋亡，这是通过抑制 c-Jun NH(2)-末端激酶 (JNK1) 的活性来实现的，从而使 Serpin B3 参与与应激反应相关的细胞过程。该蛋白与 MAPK8/JNK1 的相互作用进一步强调了其在信号通路中的复杂参与，突出了其作为多方面调节剂的潜力，对免疫调节和细胞凋亡调节具有影响。

Human

HEK293

C-6*His

P29508 (M1-P390)

6317  [NCBI]

MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQIKKVLHFDQVTENTTGKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYLFLQEYLDAIKKFYQTSVESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGNIGSNTTLVLVNAIYFKGQWEKKFNKEDTKEEKFWPNKNTYKSIQMMRQYTSFHFASLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETRVDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP

41-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 0.02% Tween80, 4% Mannitol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.